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Reaction buffers for photo reactive dyes

 

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Reaction buffers for combining with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)

One of the critical steps in all viable cell detection workflow by the means of PMA or EMA photoreaction, is sample and reagent incubation on darkness. During this treatment is very important to maximize reagent diffusion trough dead cells, spores, and cysts of microbial aggregates.

On the other hand also is necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance needs to be under control. GenIUL introduces three different reaction buffers: Standard , Standard Plus and Anaerobic.

These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.

 

Product Name
Product Price
20,00 €
Sales price: 20,00 €
20,00 €
Sales price: 20,00 €
20,00 €
Sales price: 20,00 €

  1. General Specs.
  2. Unique Features

Reaction buffers for combining with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)

One of the critical steps in all viable cell detection workflow by the means of PMA or EMA photoreaction, is sample and reagent incubation on darkness. During this treatment is very important to maximize reagent diffusion trough dead cells, spores, and cysts of microbial aggregates. On the other hand also is necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance needs to be under control. GenIUL introduces three different reaction buffers: Standard , Standard Plus and Anaerobic.

These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.

Reaction Buffers
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Bifidobacterium cells supspensions with Reaction buffer anaerobic or standard, were treated with a conventional vPCR workflow. ( 50 mM of PMA during  30 min, PhAST Blue at 100% during 15 min).
Reaction buffer anaerobic protects anaerobic cells during  vPCR work flow.

 

Thermaly inactivated (95ºC, 30 min) Mycobacteria suspension.
Untreated with PMA or treated with 50 mM, 30 min.
with Reaction Buffer standard or Reaction Buffer plus.
PhAST Blue photoactivation 10 min, 100%.
Reaction Buffer Plus completely reduces qPCR signal
Reaction Buffer standard For general purposes, neutral pH, standard ionic strength.
Reaction Buffer plus Contains anti clumping agents, cell membrane fluidising agents and a reduced level of nutrients. Designed for stabilizing live cells but accelerating reagent diffusion trough dead cells. Compatible with Enterobacteria and Mycobacteria.
Reaction Buffer anaerobic Contains anti clumping agents, minimum nutrient levels and provides a reducing environment compatible with PMA or EMA based reagents. Designed for stabilizing anaerobic live cells but accelerating reagent diffusion trough dead cells. Compatible with Bifidobacteriaceae

 

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