Reaction buffers for combining with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)
One of the critical steps in all viable cell detection workflow by the means of PMA or EMA photoreaction, is sample and reagent incubation on darkness. During this treatment is very important to maximize reagent diffusion trough dead cells, spores, and cysts of microbial aggregates.
On the other hand also is necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance needs to be under control. GenIUL introduces three different reaction buffers: Standard , Standard Plus and Anaerobic.
These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.